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HOME > Research > Equipment > Equipment List > Confocal Microscope

Olympus FluoView™ 300 Confocal Microscope

The Olympus FluoView 300 established in the microscopy suite of the Rix Building is a point-scanning, point-detection, confocal laser scanning microscope designed for biology research applications. It provides excellent resolution, efficiency of excitation and intuitive user interface. The FluoView 300 configuration permits simultaneous collection in 3 detection channels.

• Confocal microscopy can improve conventional fluorescence images by recording fluorescence generated from the focal plane within the sample, while rejecting all other light coming from above or below the focal plane. The efficient point-scan/pinhole-detection confocal optics of the FluoView systems virtually eliminate out of focus light to produce high-contrast images with superb resolution.
• The FluoView systems are fully integrated workstations that incorporate user-friendly image acquisition and image analysis software with high-resolution confocal optics that require no user alignment.
• An intuitive, Windows-based graphic user interface allows new users to quickly generate images in various scan modes, such as:
  • XY- Acquires a single confocal optical image. 360° Image rotation is available.
  • XZ- Acquires a single cross section image that cannot be obtained with a conventional microscope. The cross section image may also be rotated 360° or drawn as a free line (free line-Z).
  • XT- Acquires a single line image over time for time-lapse analysis with high temporal resolution. The cross section image may also be rotated 360° or drawn as a free line (free line-T).
  • XYZ- Acquires a series of confocal optical XY images through the thickness of the sample.
  • XYT- Acquires a single XY confocal optical image over time, at an interval that can be arbitrarily chosen. Permits observation and analysis of live cell kinetics, such as changes in intracellular calcium, pH, etc.
  • XYZT- Acquires an XYZ series over time, permitting observation and analysis of 3-dimensional changes over time in live cells.
  • Point scanning: Acquires a series of intensity changes at a single point within the image with the highest temporal resolution.
  • Automated Scanning Stage: Multi-location image acquisition is available for most imaging modes, including XYZ, XYT and XYZT, with addition of an optional automated scanning stage.
  • Standard image formats, including TIFF and AVI, permit easy, direct export of FluoView images to off-line analysis packages.
• XY scanning is performed with a pair of galvanometric mirrors, yielding a wide scanning range to cover up to a field number of 20. The optical zoom (up to 10x magnification) can be performed by narrowing the scanning range while maintaining the maximum pixel resolution of up to 2048 x 2048 pixels.
• The FluoView software, allows 0.01 micron Z-resolution via an internal stepper motor.
• Fluorescence passes through a single confocal aperture, with five distinct aperture diameters available. Based on the objective magnification and numerical aperture, the optimal aperture diameter is indicated in the software.
• Brightfield and DIC images can be simultaneously recorded with the confocal fluorescence images, and the non-confocal transmitted light images, can be superimposed to the confocal image.
• The FluoView 300 can perform time-lapsed recording at 4 frames/sec at 512 x 512, and line scan intervals at 0.5msec per line. Kinetic and ratiometric analysis is available for quantitation of fluorescence changes in live cells over time. Real time intensity plotting and ratiometric image display are also possible.
• Multi-parameter experiments can be quickly designed and executed through PAPP (Programmable Acquisition Protocol Processor) software, an integrated component of the FluoView software. With the PAPP software, advanced time-lapse protocols involving photobleaching, photoactivation or multi-location acquisition can be easily generated and saved as routine protocols.
• TTL I/O signals can be generated to coordinate experiments timed with external instrumentation.

Laser choices:

• Multi-line Argon (457nm, 488nm, 514nm) laser
• Green Helium Neon (543nm) laser
• Red Helium Neon (633nm) laser

Contact

Research Coordinator:

Phone: 250-728-3301 ext 255

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